![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3454039:Design
Designed by: Ruijie Xiang Group: iGEM20_ShanghaiTech_China (2020-10-26)
AsCas12a_RVR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 886
Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3855
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 196
Illegal PstI site found at 3667
Illegal PstI site found at 3865
Illegal NgoMIV site found at 3399
Illegal AgeI site found at 2296
Illegal AgeI site found at 2767 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1039
Illegal BsaI.rc site found at 2701
Illegal SapI.rc site found at 289
Design Notes
This is designed with MBP and his tag that can help AsCas12a mutant expression, folding and purification.
Source
It comes from expression of plasmid DNA(contains designed mutation ) of E.coli.
References
Tóth E, Varga É, Kulcsár PI, et al. Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases. Nucleic Acids Res. 2020;48(7):3722-3733. doi:10.1093/nar/gkaa110